Peptide Reconstitution Tutorial
Learn the proper technique for reconstituting lyophilized peptides with bacteriostatic water. Follow these steps carefully to ensure optimal peptide stability and effectiveness.
Research Use Only
This tutorial is for educational and research purposes only. Always consult with a qualified healthcare professional before handling or using any research peptides. Improper reconstitution can compromise peptide integrity.
1
Gather Your Supplies
Before you begin, ensure you have all necessary materials in a clean workspace:
- Peptide vial (lyophilized powder)
- Bacteriostatic water vial
- Sterile syringe with needle (typically 1ml or 3ml)
- Alcohol wipes
- Clean, flat surface
2
Clean the Vial Stoppers
Proper sterilization is critical to prevent contamination:
- Wash your hands thoroughly or wear medical gloves
- Use an alcohol wipe to clean the rubber stopper of both the peptide vial and bacteriostatic water vial
- Allow the alcohol to air dry completely (about 30 seconds)
- Do not touch the cleaned stoppers with your fingers
3
Draw Bacteriostatic Water
Carefully measure the correct amount of bacteriostatic water:
- Remove the needle cap from your sterile syringe
- Insert the needle into the bacteriostatic water vial
- Invert the vial and slowly pull back the plunger to draw the desired amount (e.g., 1ml, 2ml)
- Check the measurement markings on the syringe to ensure accuracy
- Remove any air bubbles by gently tapping the syringe and pushing the plunger slightly
4
Inject Water into Peptide Vial
This is the most critical step - inject slowly and carefully:
- Insert the needle into the peptide vial at a slight angle
- Aim the water stream at the side of the glass vial, NOT directly onto the powder
- Inject the water very slowly to avoid foaming
- The powder should dissolve naturally as the water runs down the side
- Do NOT shake the vial - this can damage the peptide structure
CRITICAL: Never inject water directly onto the powder or shake the vial. This can denature the peptide and render it ineffective.
5
Gently Dissolve the Peptide
Allow the peptide to dissolve naturally with minimal agitation:
- After injecting the water, gently swirl the vial in a circular motion
- Do NOT shake vigorously - use only gentle rotation
- The solution should become clear within 1-5 minutes
- If powder remains, let the vial sit in the refrigerator for 10-15 minutes, then gently swirl again
- Once fully dissolved, the solution should be clear and free of particles
Storage and Handling
Important information for maintaining peptide stability
Before Reconstitution
- • Store lyophilized peptides in the freezer (-20°C or colder)
- • Keep away from light and moisture
- • Most peptides remain stable for 1-2 years when frozen
- • Allow vial to reach room temperature before opening
After Reconstitution
- • Store in refrigerator (2-8°C) immediately after reconstitution
- • Most peptides remain stable for 2-4 weeks when refrigerated
- • Protect from light (wrap in foil if necessary)
- • Never freeze reconstituted peptides
- • Label vial with reconstitution date and concentration
Common Mistakes to Avoid
❌
Shaking the vial
This can denature the peptide. Use gentle swirling only.
❌
Injecting water directly onto powder
Aim at the side of the vial to prevent foaming.
❌
Using regular water
Always use bacteriostatic water to prevent bacterial growth.
❌
Freezing reconstituted peptides
This can damage the peptide structure. Refrigerate only.
Need help calculating the correct dosage after reconstitution?
Use Our Dosage Calculator